Evaluation of Organic and Vermicompost-based Trichoderma harzianum Formulation for Seedling Disease Management and Yield Enhancement in Chickpea (Cicer arietinum L.)
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Abstract
Seedling diseases caused by soil-borne pathogens pose a significant threat to chickpea (Cicer arietinum L.) production, often resulting in poor plant stand, reduced growth, and substantial yield losses. Eco-friendly and sustainable disease management strategies are therefore essential to minimize chemical inputs while enhancing soil and plant health. In this study, organic composts and vermicompost were screened as carrier materials for the formulation of Trichoderma harzianum, an effective biocontrol agent, to evaluate their efficacy in managing seedling diseases caused by soil-borne pathogens, Sclerotium rolfsii and Fusarium oxysporum f. sp. ciceri, and promoting growth and yield of chickpea. The experiments were conducted in the fields of the Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur, during 2018-19, 2019-20, and 2020-21 cropping years to evaluate the antagonistic potential of organic composts and vermicompost-based T. harzianum formulations designated as Tricho-organic-compost and Tricho-vermi-compost on seedling emergence, disease incidence, plant growth, and grain yield of chickpea. Seed treatment with fungicide Provax 200 WP was also tested. Results revealed that T. harzianum enriched organic composts and vermicompost, viz. Tricho-organic-compost and Tricho-vermi-compost soil, seed treatment by Provax 200 WP, and soil amendment with organic-compost and vermi-compost were effective in reducing seedling mortality and increasing plant growth and yield of chickpea compared to the control. Among the treatments, T. harzianum enriched organic composts and vermicompost, viz. Tricho-organic-compost and Tricho-vermi-compost exhibited superior for significant reduction in seedling mortality, and enhancement in plant growth parameters, and increased yield of chickpea compared to untreated control and other treatments. The study underscores the potential of compost-based T. harzianum formulations as sustainable tools for integrated management of chickpea seedling diseases while enhancing crop productivity. Seed treatment with the chemical fungicide Provax 200 WP was also a better treatment for managing seedling disease and increasing plant growth and yield of chickpea.
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Vishwadhar, Gurha SN. Integrated management of chickpea diseases. In: Chamola, Dubey OP, editors. New Delhi (India): ABH Publishing Co. 1998;249.
Hasanuzzaman M, Karim MF, Fattah QA, Nahar K. Yield performance of chickpea varieties following application of growth regulator. Am-Euras J Sci Res. 2007;2(2):117–120. Available from:
https://www.idosi.org/aejsr/2(2)07/8.pdf
Xing Q, Dekker S, Kyriakopoulou K, Boom RM, Smid EJ, Schutyser MA. Enhanced nutritional value of chickpea protein concentrate by dry separation and solid-state fermentation. Innov Food Sci Emerg Technol. 2020;59:102269. Available from: https://doi.org/10.1016/j.ifset.2019.102269
Jambhulkar PP, Sharma P, Meghwal M. Additive effect of soil application with Trichoderma-enriched FYM along with seed treatment and drenching with Trichoderma formulation for management of wet root rot caused by Rhizoctonia solani in chickpea. J Pure Appl Microbiol. 2015;9(1):1–8. Available from:
https://www.researchgate.net/publication/282265582
Arriagada O, Cacciuttolo F, Cabeza RA, Carrasco B, Schwember AR. A comprehensive review on chickpea (Cicer arietinum L.) breeding for abiotic stress tolerance and climate change resilience. Int J Mol Sci. 2022;23(12):6794. Available from: https://doi.org/10.3390/ijms23126794
Zhang J, Wang J, Zhu C, Singh RP, Chen W. Chickpea: its origin, distribution, nutrition, benefits, breeding, and symbiotic relationship with Mesorhizobium species. Plants. 2024;13(3):429. Available from: https://doi.org/10.3390/plants13030429
Nene Y. Diseases of chickpea. Presented at the Proceedings of the International Workshop on Chickpea Improvement; 1979 Feb 28–Mar 2; Hyderabad. Available from: https://oar.icrisat.org/764/1/RA_00042.pdf
Fakir GA. Status of research on pulse disease at the Bangladesh Agricultural University (BAU). Mymensingh: Department of Plant Pathology, BAU. 1983;19. Available from: https://www.scirp.org/reference/referencespapers?referenceid=3146396
Bakr MA, Ahmed HU, Ahmed MA, Wadud Mian MA. Advances in plant pathological research in Bangladesh. Presented at the National Workshop on Strategic Intervention on Plant Pathological Research in Bangladesh. Plant Pathology Division. 2007;344.
Kukreja S, Salaria N, Thakur K, Goutam U. Fungal disease management in chickpea: current status and prospects. In: Fungi and Their Role in Sustainable Development: Current Perspectives. Singapore: Springer; 2018;293–309. Available from:
https://doi.org/10.1007/978-981-13-0393-7_17
Azhar H, Muhammad Iqbal SH, Najma A, Zahid AM. Factors affecting the development of collar rot disease in chickpea. Pak J Bot. 2006;38(1):211–216. Available from:
https://www.researchgate.net/publication/281203706
Begum F. Integrated control of seedling mortality of lentil caused by Sclerotium rolfsii [master’s thesis]. Gazipur (Bangladesh): Bangladesh Sheikh Mujibur Rahman Agricultural University. 2003.
Hashem A, Tabassum B, Abd_Allah EF. Omics approaches in chickpea Fusarium wilt disease management. In: Singh B, Singh G, Kumar K, Nayak S, Srinivasa N, editors. Management of fungal pathogens in pulses. Cham: Springer; 2020. Available from:
https://doi.org/10.1007/978-3-030-35947-8_4
Kirkegaard JA, Sarwar M, Wong PTW, Mead A, Howe G, Newell M. Field studies on the biofumigation of take-all by Brassica break crops. Aust J Agric Res. 2000;51(4):445–456. Available from:
https://doi.org/10.1071/AR99106
Ryckeboer J. Biowaste and yard waste composts: microbiological and hygienic aspects: suppressiveness to plant diseases [dissertation]. Leuven (Belgium): Katholieke Universiteit Leuven; 2001. Available from: https://www.researchgate.net/publication/35683329
Bailey KL, Lazarovits G. Suppressing soilborne diseases with residue management and organic amendments. Soil Tillage Res. 2003;72(2):169–180. Available from:
https://doi.org/10.1016/S0167-1987(03)00086-2
Louws FJ, Rivard CL, Kubota C. Grafting fruiting vegetables to manage soilborne pathogens, foliar pathogens, arthropods, and weeds. Sci Hortic. 2010;127(2):127–146. Available from:
https://doi.org/10.1016/j.scienta.2010.09.023
Haque Z, Nawaz S, Haidar L, Ansari MSA. Development of novel Trichoderma bioformulations against Fusarium wilt of chickpea. Sci Rep. 2025;15(1):1–9.
Hugar A, Nayaka S. Trichoderma harzianum isolate AKH-5 enhances defense response in Cajanus cajan (L.) Millsp. against Fusarium oxysporum f. sp. udum and promotes plant growth. Microbe. 2025;8:1–15. Available from: https://doi.org/10.1038/s41598-025-86984-y
Nirmalkar VK, Singh S, Tiwari RS, Said PP, Kaushik DK. Field efficacy of Trichoderma harzianum and Rhizobium against wilt complex of chickpea. Int J Curr Microbiol App Sci. 2017;6(7):1421–1429. Available from: https://doi.org/10.20546/ijcmas.2017.607.170
Kumari R, Kumar V, Koul B, Farah MA, Mishra AK. Synergistic effects of Trichoderma and biochar on chickpea growth and disease reduction. Front Microbiol. 2025;1:1–16. Available from:
https://doi.org/10.3389/fmicb.2025.1583114
Unsoed P, Yuwariah Y, Sumsadi S, Simarmata T. Viability of T. harzianum grown on different carrier formulations. KnE Life Sci. 2017;2(6):95. Available from: https://doi.org/10.18502/kls.v2i6.1024
Jyoti, Khwairakpam M. Microbial fortification of rotary drum compost and vermicompost with Trichoderma viride via solid-state fermentation and GC-MS profiling of bioactive compounds. Discov Appl Sci. 2025;7:1–18. Available from:
https://doi.org/10.1007/s42452-025-07387-2
Sahni S, Sarma BK, Singh DP, Singh HG, Singh KP. Vermicompost enhances the performance of plant growth-promoting rhizobacteria in Cicer arietinum rhizosphere against Sclerotium rolfsii. Crop Prot. 2008;27(3–5):369–76. Available from:
https://doi.org/10.1016/j.cropro.2007.07.001
Jyoti, Khwairakpam M. Synergistic effect of Trichoderma viride-enriched rotary drum compost and vermicompost extracts against fungal phytopathogens. Bioresour Technol Rep. 2025;30:1–12. Available from: https://doi.org/10.1016/j.biteb.2025.102156
Baxter AP, Rong IH, Roux C, Van der Linde EJ. Collecting and Preserving Fungi Manual for Mycology. Pretoria (South Africa): Plant Protection Research Institute; 1999. Available from: https://assets.ippc.int/static/media/uploads/resources/collecting_and_preserving_fungi.pdf
Barnett HL, Hunter BB. Illustrated Genera of Imperfect Fungi. 3rd ed. Minneapolis (USA): Burges Co.; 1972. Available from: https://www.scirp.org/reference/referencespapers?referenceid=1287870
Booth C. The Genus Fusarium. Kew, Surrey (England): Commonwealth Mycology Institute; 1971. Available from: https://www.scirp.org/reference/referencespapers?referenceid=1257093
Dubey SC, Suresh M, Singh B. Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biol Control. 2007;40(1):118–27. Available from:
https://doi.org/10.1016/j.biocontrol.2006.06.006
Sultana N, Ghaffar A. Effect of organic amendments and Trichoderma harzianum on the growth and root rot disease complex of soybean. Pak J Bot. 2010;42(5):373–83.
Hossain I, Fakir GA. Biological Control of Seed-Borne Pathogens of Some Crops. 1st ed. Mymensingh (Bangladesh): Seed Pathology Laboratory, Bangladesh Agricultural University; 2001.
Arancon NQ, Edwards CA, Bierman P. Influences of vermicomposts on field strawberries: Part 2. Effects on soil microbiological and chemical properties. Bioresour Technol. 2004;93(2):215–20. Available from:
https://doi.org/10.1016/j.biortech.2003.10.014
Bokhtiar SM, Sakurai K. Effects of organic manure and chemical fertilizer on soil fertility and productivity of plant and ratoon crops of sugarcane. Arch Agron Soil Sci. 2005;51(3):325–34. Available from:
https://doi.org/10.1080/03650340500098006
Bhat NA, Anwar A, Hussain A. Effect of organic manures and biofertilizers on growth, yield, and quality of tomato (Lycopersicon esculentum Mill.). Afr J Biotechnol. 2013;12(7):684–89.
Mishra DS, Sinha AP. Plant growth-promoting activity of some fungal and bacterial agents on rice seed germination and seedling growth. Trop Agric. 2000;77:188–91. Available from: https://www.researchgate.net/publication/289205425_Plant_growth-promoting_activity_of_some_fungal_and_bacterial_agents_on_rice_seed_germination_and_seedling_growth
Prasad D, Anes KM. Effect of metabolites of Trichoderma harzianum and T. viride on plant growth and Meloidogyne incognita on okra. Ann Plant Prot Sci. 2008;16:461–65. Available from:
https://www.ijour.net/article/apps-16-2-049
Mukhtar I. Influence of Trichoderma species on seed germination in okra. Mycopath. 2008;6(1&2):47–50. Available from: https://www.researchgate.net/publication/242591669_Influence_of_Trichoderma_species_on_seed_germination_in_okra
Sharma P, Sharma M, Srinivas C. Enhancement of biocontrol potential of Trichoderma harzianum through UV mutagenesis for management of chickpea wilt. J Plant Pathol. 2014;96(1):101–7. Available from: https://www.researchgate.net/publication/282705869_Biocontrol_efficiency_of_Trichoderma_harzianum_against_chickpea_wilt_pathogen_Fusarium_oxysporum
Singh A, Shukla A, Kumar V. Eco-friendly management of plant pathogens through bio-agents and organic amendments. Int J Bioresour Stress Manag. 2016;7(3):507–14.
Nene YL, Reddy MV. Chickpea diseases and their control. In: The Chickpea. Wallingford (UK): C.A.B. International. 1987;233–70.
Poddar RK, Singh DV, Dubey SC. Integrated application of Trichoderma harzianum mutants and carbendazim to manage chickpea wilt (Fusarium oxysporum f.sp. ciceri). Indian J Agric Sci. 2004;74:346–48. Available from: https://www.researchgate.net/publication/279550471_Integrated_application_of_Trichoderma_harzianum_mutants_and_carbendazim_to_manage_chickpea_wilt_Fusarium_oxysporum_f_sp_ciceri
Rojo FG, Reynoso MM, Sofia MF, Chulze N, Torres AM. Biological control by Trichoderma species of Fusarium solani causing peanut brown root rot under field conditions. Crop Prot. 2007;26:549–55. Available from: https://doi.org/10.1016/j.cropro.2006.05.006
Nahar MS, Rahman MA, Miller. Use of Tricho-compost and Tricho-leachate for management of soil-borne pathogens and production of healthy cabbage seedlings. Bangladesh J Agric Res. 2012;37:653–64. Available from: https://doi.org/10.3329/bjar.v37i4.14390
Uzun I. Use of spent mushroom compost in sustainable fruit production. J Fruit Ornam Plant Res. 2004;12:157–65. Available from:
https://www.researchgate.net/publication/228703372
Younis NA. Mycoparasitism of Trichoderma harzianum and Trichoderma longibrachiatum on Fusarium oxysporum f.sp. phaseoli is the cause of bean wilt disease. Bull Fac Agric Cairo Univ. 2005;56:201–19.
Azarmi R, Hajieghrari B, Giglou A. Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake. Afr J Biotechnol. 2011;10(31):5850–5. Available from: https://doi.org/10.5897/AJB10.1600
Harman GE, Herrera-Estrella AH, Horwitz BA, Lorito M. Special issue: Trichoderma – from basic biology to biotechnology. Microbiology. 2012;158:1–2. Available from: https://doi.org/10.1099/mic.0.056424-0
Hermosa R, Viterbo A, Chet I, Monte E. Plant-beneficial effects of Trichoderma and of its genes. Microbiology. 2012;158:17–25. Available from: https://doi.org/10.1099/mic.0.052274-0
Samolski I, Rincon AM, Pinzon LM, Viterbo A, Monte E. The qid74 gene from Trichoderma harzianum has a role in root architecture and plant biofertilization. Microbiology. 2012;158:129–38. Available from:
https://doi.org/10.1099/mic.0.053140-0
Kumar S, Kaushik N, Sharma R. Development of bioformulation of Trichoderma harzianum for integrated management of collar rot of chickpea. J Plant Pathol. 2017;99(2):361–9.
Chowdhury MSM, Hossain I, Fakir GA, Aminuzzaman FM, Islam MR. Tolerance of Trichoderma harzianum and Gliocladium viride to agrochemicals and their antagonistic effect on seed-borne mycoflora of pigeon pea. Bangladesh J Seed Sci Technol. 2000;4(1&2):83–6. Available from: http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/4314
Hossain I, Shamsuzzaman SM. Developing Trichoderma-based bio-fungicide using agro-waste. BAU Res Prog. 2003;14:49–50.
Yeasmin R. Integrated management of seedling diseases of blackgram, mungbean, and lentil [MSc thesis]. Mymensingh (Bangladesh): Department of Plant Pathology, Bangladesh Agricultural University. 2004;72–3.
Hossain I, Naznin MHA. BAU biofungicide in controlling seedling disease of some summer vegetables. BAU Res Progr. 2005;15:32–5.
Rahman R. Trichoderma fortified compost in controlling diseases and increasing the yield of tomato [MSc thesis]. Gazipur (Bangladesh): Bangabandhu Sheikh Mujibur Rahman Agricultural University; 2013. Available from: https://doi.org/10.22161/ijeab.91.17
Mohiddin FA, Bashir I, Padder SA, Hamid B. Evaluation of different substrates for mass multiplication of Trichoderma species. J Pharmacogn Phytochem. 2017;6(6):563–9. Available from: https://www.phytojournal.com/archives/2017/vol6issue6/PartI/6-5-512-559.pdf
Sajad UN, Wasim HR, Dar MS, Kirmani SN, Mudasir M. New generation fungicides in disease management of horticultural crops. Hortic J. 2017;7(1):1–7. Available from: