Published: 10 November, 2021 | Volume 5 - Issue 2 | Pages: 028-031
Representative FISH Panel probes. The first eight cells each with two boxes marked with the number 1-2-3-4 show the normal ch.11 (left box) and the der(17) (right box) hybridized with different probes as follow: Box 1: WT1/FLI1 by combination probe set. FISH result highlight the der(17) orientation: green signal (WT1) map on the short arm of chromosome 11 and orange signal (FLI1) map on the long arm of chromosome 11. Box 2: CCND1 Dual Color Break Apart Probe Rearrangement. Der(17) harboring the CCND1 gene in closed proximity to 17p compared with normal chromosome. Box 3 LSI ATM/CEP11. As shown on normal ch.11 (left box) the orange fluorochrome hybridizes ATM gene and the green fluorochrome direct labeled the CEP11. The star on der(17) (right box) show the interstitial deletion affecting the gene ATM and the loss of CEP11. Box 4: MLL Dual Color Break Apart rearrangement. Der(17) harboring the MLL gene in closed proximity to 11p. The last two boxes, marked with the number 5, shows the normal ch.17 (left box) and der(17) in the (right box) hybridized with TP53 (orange)/CEP17(green). As illustrated der(17) keeps intact the CEP17.